In-vitro metabolomics to evaluate toxicity of particulate matter under environmentally realistic conditions.

摘要: Abstract In this pilot study three fractions of particulate matter (PM0.25, PM2.5-0.25, and PM10-2.5) were collected in environments (classroom, home, outdoors) a village located nearby an industrial complex. Time-activity pattern 20 students attending the classroom was obtained, dose particles reaching children's lungs under actual environmental conditions (i.e. real dose) calculated via dosimetry model. The highest PM concentrations reached classroom. Simulations showed that heavy intensity outdoor activities played major role deposition, especially upper part respiratory tract. mass PM10-2.5 alveoli minor, while PM2.5-0.25 PM0.25 apportion for most retained lungs. Consequently, only used two subsequent toxicity assays onto alveolar cells (A549). First, cytotoxicity dose-response assay performed, doses corresponding to 5% mortality (LC5) estimated. Afterwards, LC-MS metabolomic conducted: one applying LC5, another dose. A lower estimated LC5 value obtained than (8.08 73.7 ng/mL respectively). number altered features after exposure similar both (39 38 respectively), these numbers differed (10 5 metabolic changes related membrane lung surfactant lipids. This highlights capacity alter profile at conventional levels.