Identification and functional characterization of microRNAs involved in the malignant progression of gliomas.

摘要: Diffuse astrocytoma of World Health Organization (WHO) grade II has an inherent tendency to spontaneously progress anaplastic WHO III or secondary glioblastoma IV. We explored the role microRNAs (miRNAs) in glioma progression by investigating expression profiles 157 miRNAs four patients with primary gliomas that progressed IV glioblastomas. Thereby, we identified 12 (miR-9, miR-15a, miR-16, miR-17, miR-19a, miR-20a, miR-21, miR-25, miR-28, miR-130b, miR-140 and miR-210) showing increased expression, two (miR-184 miR-328) reduced upon progression. Validation experiments on independent series low-grade high-grade astrocytomas confirmed miR-17 miR-184 as promising candidates, which were selected for functional analyses. These studies revealed miRNA-specific influences viability, proliferation, apoptosis invasive growth properties A172 T98G cells vitro. Using mRNA protein profiling, distinct sets transcripts proteins differentially expressed after inhibition overexpression cells. Taken together, our results support important altered miRNA gliomas, suggest interesting candidates contributing