Cell culture adaptation of Puumala hantavirus changes the infectivity for its natural reservoir, Clethrionomys glareolus, and leads to accumulation of mutants with altered genomic RNA S segment.

摘要: This paper reports the establishment of a model for hantavirus host adaptation. Wild-type (wt) (bank vole-passaged) and Vero E6 cell-cultured variants Puumala virus strain Kazan were analyzed their virologic genetic properties. The wt variant was well adapted reproduction in bank voles but not cell culture, while strains replicated to much higher efficiency culture did reproducibly infect voles. Comparison consensus sequences respective viral genomes revealed no differences coding region S gene. However, noncoding regions gene found be different at positions 26 1577. In one additional independent adaptation experiment, all cDNA clones from E6-adapted carry substitutions position 1580 segment, just 3 nucleotides downstream mutation observed first No entire M segments variants. results indicated impacts genomic process selective advantages that carried altered segment. We conclude isolation resulted phenotypically genotypically hantavirus.