Co-delivery of as-miR-21 and 5-FU by poly(amidoamine) dendrimer attenuates human glioma cell growth in vitro.
作者： Yu Ren , Chun-Sheng Kang , Xu-Bo Yuan , Xuan Zhou , Peng Xu
关键词: Cell growth 、 Glioma 、 Cytotoxicity 、 Molecular biology 、 Antisense therapy 、 Poly(amidoamine) 、 Cancer research 、 Materials science 、 MTT assay 、 Apoptosis 、 Cell culture
摘要: MicroRNAs have been demonstrated to be deregulated in different types of cancer. miR-21 is a key player in the majority of cancers. Down-regulation of miR-21 in glioblastoma cells leads to repression of cell growth, increased cellular apoptosis and cell-cycle arrest, which can theoretically enhance the chemotherapeutic effect in cancer therapy. In this study, the poly (amidoamine)(PAMAM) dendrimer was employed as a carrier to co-deliver antisense-miR-21 oligonucleotide (as-miR-21) and 5-fluorouracil (5-FU) to achieve delivery of as-miR …
sci-hub.se 参考文章(18)
David P Bartel, MicroRNAs: Genomics, Biogenesis, Mechanism, and Function Cell. ,vol. 116, pp. 281- 297 ,(2004) , 10.1016/S0092-8674(04)00045-5
Fanyin Meng, Roger Henson, Hania Wehbe–Janek, Kalpana Ghoshal, Samson T. Jacob, Tushar Patel, MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer Gastroenterology. ,vol. 133, pp. 647- 658 ,(2007) , 10.1053/J.GASTRO.2007.05.022
Volker Wacheck, Doris Losert, Patrick Günsberg, Hans-Peter Vornlocher, Philipp Hadwiger, Anke Geick, Hubert Pehamberger, Markus Müller, Burkhard Jansen, Small interfering RNA targeting bcl-2 sensitizes malignant melanoma. Oligonucleotides. ,vol. 13, pp. 393- 400 ,(2003) , 10.1089/154545703322617078
Shuomin Zhu, Min-Liang Si, Hailong Wu, Yin-Yuan Mo, MicroRNA-21 targets the tumor suppressor gene tropomyosin 1 (TPM1) Journal of Biological Chemistry. ,vol. 282, pp. 14328- 14336 ,(2007) , 10.1074/JBC.M611393200
Jean Haensler, Francis C. Szoka, Polyamidoamine cascade polymers mediate efficient transfection of cells in culture Bioconjugate Chemistry. ,vol. 4, pp. 372- 379 ,(1993) , 10.1021/BC00023A012
I A Asangani, S A K Rasheed, D A Nikolova, J H Leupold, N H Colburn, S Post, H Allgayer, MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Oncogene. ,vol. 27, pp. 2128- 2136 ,(2008) , 10.1038/SJ.ONC.1210856
Li Qin, Xing Zhang, Ling Zhang, Yan Feng, Gui-Xiang Weng, Man-Zhi Li, Qing-Li Kong, Chao-Nan Qian, Yi-Xin Zeng, Mu-Sheng Zeng, Duan-Fang Liao, Li-Bing Song, Downregulation of BMI-1 enhances 5-fluorouracil-induced apoptosis in nasopharyngeal carcinoma cells. Biochemical and Biophysical Research Communications. ,vol. 371, pp. 531- 535 ,(2008) , 10.1016/J.BBRC.2008.04.117
Benjamin P. Lewis, Christopher B. Burge, David P. Bartel, Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets Cell. ,vol. 120, pp. 15- 20 ,(2005) , 10.1016/J.CELL.2004.12.035
István J. Majoros, Thommey P. Thomas, Chandan B. Mehta, James R. Baker, Poly(amidoamine) Dendrimer-Based Multifunctional Engineered Nanodevice for Cancer Therapy Journal of Medicinal Chemistry. ,vol. 48, pp. 5892- 5899 ,(2005) , 10.1021/JM0401863
D. Bhadra, S. Bhadra, S. Jain, N.K. Jain, A PEGylated dendritic nanoparticulate carrier of fluorouracil. International Journal of Pharmaceutics. ,vol. 257, pp. 111- 124 ,(2003) , 10.1016/S0378-5173(03)00132-7