Genomic and Transcriptomic Analyses of the Facultative Methanotroph Methylocystis sp. Strain SB2 Grown on Methane or Ethanol

摘要: A minority of methanotrophs are able to utilize multicarbon compounds as growth substrates in addition methane. The pathways utilized by these microorganisms for assimilation compounds, however, have not been explicitly examined. Here, we report the draft genome facultative methanotroph Methylocystis sp. strain SB2 and perform a detailed transcriptomic analysis cultures grown with either methane or ethanol. Evidence use canonical oxidation pathway serine cycle carbon from was obtained, well operation complete tricarboxylic acid (TCA) ethylmalonyl-coenzyme (EMC) pathway. Experiments on revealed that genes responsible first step oxidation, conversion methanol, were expressed at significantly higher level than those downstream oxidative transformations, suggesting this may be rate limiting Further, analyses ethanol compared (i) expression reduced, (ii) TCA dramatically increased, (iii) EMC similar. Based data, it appears converts acetyl-coenzyme A, which is then funneled into energy generation incorporated biomass via This suggests some greater metabolic flexibility previously thought multiple highly controlled integrated.