Effects of release factor context at UAA codons in Escherichia coli.

作者： R Martin , M Weiner , J Gallant

DOI: 10.1128/JB.170.10.4714-4717.1988

关键词: Biology 、 Gene 、 Context (language use) 、 Release factor 、 Peptide Termination Factors 、 Stop codon 、 Genetics 、 Escherichia coli 、 Transfer RNA 、 Plasmid

摘要: In Escherichia coli, nonsense suppression at UAA codons is governed by the competition between a suppressor tRNA and translational release factors RF1 RF2. We have employed plasmids carrying genes for RF2 to measure factor preference 13 different sites in lacI gene. show here that activity of varies according messenger context. favored which are efficiently suppressed. preferred poorly suppressed sites.