Spatially clustered neuronal assemblies comprise the microstructure of synchrony in chronically epileptic networks

摘要: Epilepsy is characterized by recurrent synchronizations of neuronal activity, which are both a cardinal clinical symptom and debilitating phenomenon. Although the temporal dynamics epileptiform well described at macroscopic level using electrophysiological approaches, less known about how spatially distributed microcircuits contribute to these events. It important understand relationship between micro macro network activity because various mechanisms proposed underlie generation such pathological united assumption that epileptic hypersynchronous across multiple scales. However, quantitative analyses spatial with cellular resolution have been hampered difficulty simultaneously recording from neurons in lesioned, adult brain tissue. We overcome this experimental limitation used two-photon calcium imaging combination functional clustering algorithm uncover structure chronically dentate gyrus mouse pilocarpine model lobe epilepsy. show that, under hyperexcitable conditions, slices display interictal-like events high diversity patterns individual neurons. Analysis reveals clusters localized comprise networks, composed coactivation variable subsets clusters, little repetition Thus, not necessarily when viewed microcircuit scale instead patterned but structure.