High amplitude direct compressive strain enhances mechanical properties of scaffold-free tissue-engineered cartilage.

摘要: Adult cartilage has a limited healing capacity. Damages resulting from disease or injury increase over time and cause severe pain. One approach to reinstate the function is tissue engineering (TE). However, generation of TE consuming expensive its properties are so far suboptimal. As in vivo subject loading, it assumed that mechanical stimulation may enhance quality cartilage. In this study short-term influence variable compressive strain amplitudes on biochemical scaffold-free was investigated. Primary porcine chondrocytes were isolated, proliferated, redifferentiated, transferred onto hydroxyapatite carriers, cartilage-carrier constructs. These constructs placed custom-made bioreactor. Compression 5%, 10%, 20% applied. each experiment four loaded with dynamic compression (3000 cycles/day, 1 Hz) for 14 days...