Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil
作者： Anna Chojnacka , Witold Gładkowski , Aleksandra Grudniewska
DOI: 10.3390/MOLECULES22101771
关键词: Cod liver oil 、 Lipase 、 Transesterification 、 Candida antarctica 、 Chromatography 、 Polyunsaturated fatty acid 、 Yolk 、 Enzyme 、 Stearidonic acid 、 Chemistry
摘要: Phospholipids containing PUFAs are important vehicles for their delivering to the targeted tissues. In our research project we established enzymatic methods enrichment of natural egg-yolk PC with n-3 PUFAs. Instead synthetic PUFA ethyl esters, new strategy was developed using polyunsaturated fatty acids enriched fraction (PUFA-EF) from cod liver oil as acyl donors. PUFA-EF produced by urea-complexation and contained 86.9% including 8.5% stearidonic acid (SDA; 18:4(n-3)), 26.7% EPA, 45.2% DHA. The transesterification catalyzed lipases. After screening enzymes effect reaction medium; molar ratio substrates etc. investigated. highest incorporation 45.6%; 36.8% DHA 5.8% EPA at following conditions: hexane; 55 °C; PUFA-EF/PC 10; 48 h time lipase B Candida antarctica a biocatalyst (20% enzyme load).
mdpi.com
core.ac.uk
sci-hub.se 参考文章(52)
Chang Won Park, Seok Joon Kwon, Jeong Jun Han, Joon Shick Rhee, Transesterification of phosphatidylcholine with eicosapentaenoic acid ethyl ester using phospholipase A2 in organic solvent Biotechnology Letters. ,vol. 22, pp. 147- 150 ,(2000) , 10.1023/A:1005626508891
M.C. Milinsk, A.E. Murakami, S.T.M. Gomes, M. Matsushita, N.E. de Souza, Fatty acid profile of egg yolk lipids from hens fed diets rich in n-3 fatty acids Food Chemistry. ,vol. 83, pp. 287- 292 ,(2003) , 10.1016/S0308-8146(03)00094-3
Eileen E Birch, Dennis R Hoffman, Ricardo Uauy, David G Birch, Claude Prestidge, Visual Acuity and the Essentiality of Docosahexaenoic Acid and Arachidonic Acid in the Diet of Term Infants Pediatric Research. ,vol. 44, pp. 201- 209 ,(1998) , 10.1203/00006450-199808000-00011
M. Ghosh, D. K. Bhattacharyya, Soy lecithin‐monoester interchange reaction by microbial lipase Journal of the American Oil Chemists' Society. ,vol. 74, pp. 761- 763 ,(1997) , 10.1007/S11746-997-0215-9
Douglas G. Hayes, Ylva C. Bengtsson, James M. Van Alstine, Fredrik Setterwall, Urea Complexation for the Rapid, Ecologically Responsible Fractionation of Fatty Acids from Seed Oil Journal of the American Oil Chemists' Society. ,vol. 75, pp. 1403- 1409 ,(1998) , 10.1007/S11746-998-0190-9
Gudmundur G. Haraldsson, Atli Thorarensen, Preparation of Phospholipids Highly Enriched with n-3 Polyunsaturated Fatty Acids by Lipase Journal of the American Oil Chemists' Society. ,vol. 76, pp. 1143- 1149 ,(1999) , 10.1007/S11746-999-0087-2
Lena Burri, Nils Hoem, Sebastiano Banni, Kjetil Berge, Marine Omega-3 Phospholipids: Metabolism and Biological Activities International Journal of Molecular Sciences. ,vol. 13, pp. 15401- 15419 ,(2012) , 10.3390/IJMS131115401
S.T. Russell, M.J. Tisdale, Effect of eicosapentaenoic acid (EPA) on expression of a lipid mobilizing factor in adipose tissue in cancer cachexia Prostaglandins Leukotrienes and Essential Fatty Acids. ,vol. 72, pp. 409- 414 ,(2005) , 10.1016/J.PLEFA.2005.03.002
Yoichiro Totani, Setsuko Hara, Preparation of polyunsaturated phospholipids by lipase-catalyzed transesterification Journal of the American Oil Chemists' Society. ,vol. 68, pp. 848- 851 ,(1991) , 10.1007/BF02660600
Michel Lagarde, Nathalie Bernoud, Nicole Brossard, Dominique Lemaitre-Delaunay, Frank Thiès, Martine Croset, Jean Lecerf, Lysophosphatidylcholine as a preferred carrier form of docosahexaenoic acid to the brain Journal of Molecular Neuroscience. ,vol. 16, pp. 201- 204 ,(2001) , 10.1385/JMN:16:2-3:201