Plasticity of ovarian cancer cell SKOV3ip and vasculogenic mimicry in vivo.

摘要: The aim of this study is to investigate the plasticity human epithelial ovarian cancer cell SKOV3ip and formation vasculogenic mimicry (VM) in vivo. was transfected with lentiviral vector carrying green fluorescence protein (GFP). Female nude mice were implanted intraperitoneally GFP-labled SKOV3ip. When transplanted tumor reached a volume approximately 1 cm3, paraffin-embedded, formaldehyde-fixed tissue prepared stained hematoxylin eosin (H & E). Tumor tissues also studied by electron microscopy microscopy. results H E staining, microscopy, indicated formed patterned networks erythrocytes them, absence vascular cells, which sign that engaged VM Expression epithelium marker CD31 investigated immunohistochemical immunofluorescence assay, semiquantitative reverse transcriptase–polymerase chain reaction (RT-PCR), flow cytometric analysis (FACS). Factor VIII endothelial growth factor (VEGF) analyzed FACS. Weak focal staining found along channels cells. Immunofluorescence assay RT-PCR demonstrated expressed primary-cultured VIII, but not VEGF detected present has shown line may be able express some specific markers cells form In following study, we hypoxia-inducible (HIF)-1α inhibitor, rapamycin, could possibly prevent phenotype transformation SKOV3ip, reflected down-regulating expression VIII. HIF-1α correlated These might associated HIF-1α.