Identification of specific outer membrane polypeptides associated with virulent Yersinia enterocolitica.

摘要: Abstract An antiserum (WA-SAA) that agglutinates specifically with mouse virulent but not avirulent strains of Yersinia enterocolitica was used to identify virulence-associated factors by Western blot techniques. Several outer membrane polypeptides were identified only in the strains, which included serotypes O:8, O:3, O:9, O:4,32, O:5,27, and O:21. These three, possibly four, major polypeptides. The prominent high-molecular-weight species demonstrated both sodium dodecyl sulfate-polyacrylamide gel electrophoresis blot, whereas others revealed technique. Expression these correlated agglutination reaction presence a 42- and/or 82-megadalton plasmid. highly temperature dependent slightly affected inclusion 10 mM Ca2+ growth medium. produced during logarithmic stationary phases at 37 degrees C. We suggest production specific calcium dependency may be coded for plasmid(s) are regulated independent mechanisms. appear novel markers Y. important pathogenicity this organism.