Simultaneous ultraperformance liquid chromatography/tandem mass spectrometry determination of four antihypertensive drugs in human plasma using hydrophilic-lipophilic balanced reversed-phase sorbents sample preparation protocol.

摘要: Therapeutic drug monitoring of angiotensin-converting enzyme inhibitors has a great impact on blood pressure control in patients with heart failure and hepatic renal impairment. To provide an efficient tool for assessment plasma, UPLC-MS/MS method was developed simultaneous determination benazepril hydrochloride, fosinopril sodium, captopril hydrochlorothiazide human plasma samples. Solid phase extraction applied sample preparation using OASIS® hydrophilic-lipophilic balanced reversed-phase sorbents cartridges. Chromatographic separation performed Agilent SB-C18 column methanol-0.1% formic acid water (95:5, v/v) as mobile phase, at flow rate 0.3 mL/min. Detection accomplished tandem mass spectrometer. The validated according to US Food Drug Administration guidelines. It showed good linearity over concentration ranges 5-400 ng/mL sodium 100-3500 ng/mL captopril. CV% values were <13.92% whereas the mean accuracy ranged from 94.50 113.82% quality samples their recoveries 90.60 99.38%. In conclusion, present study revealed selectivity sensitivity; it can be estimation angiotensin converting dose adjustment therapeutic monitoring.