The Saccharomyces cerevisiae PHM8 Gene Encodes a Soluble Magnesium-dependent Lysophosphatidic Acid Phosphatase *
作者： Venky Sreedhar Reddy , Arjun Kumar Singh , Ram Rajasekharan
关键词: Saccharomyces cerevisiae 、 Phosphatase 、 Autotaxin 、 Biology 、 Recombinant DNA 、 Yeast 、 Transmembrane domain 、 Lysophosphatidic acid 、 Biochemistry 、 Molecular biology 、 Phosphate
摘要: Phosphate is the essential macronutrient required for growth of all organisms. In Saccharomyces cerevisiae, phosphatases are up-regulated, and level lysophosphatidic acid (LPA) drastically decreased under phosphate-starved conditions. The reduction in LPA attributed to PHM8, a gene unknown function. phm8Δ yeast showed LPA-hydrolyzing activity phosphate-limiting Overexpression PHM8 resulted an increase phosphatase vivo. vitro assays purified recombinant Phm8p revealed magnesium-dependent activity, with maximal at pH 6.5. did not hydrolyze any lipid phosphates other than LPA. silico analysis suggest that soluble protein no transmembrane domain. Site-directed mutational studies aspartate residues DXDXT motif important catalysis. These findings indicated plays direct role phosphate starvation. This first report identification characterization phosphatase.
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