Enhanced metastatic potential in the MB49 urothelial carcinoma model

摘要: Recent data suggest that patients with a basal/stem-like bladder cancer (BC) subtype tend to have metastatic disease, but this is unconfirmed. Here we report the identification of murine MB49 cell line sub-clones stem-like characteristics in culture. Subcutaneous implantation S2 and S4 into immunocompetent mice resulted lung metastases 50% 80% respectively, whereas none implanted parental cells developed metastasis. Gene profiling cultured from primary tumors revealed panel genes basal/stem-like/EMT properties amplified during progression. Among them, ITGB1, TWIST1 KRT6B are consistently up-regulated both sub-clones. To evaluate clinical relevance, examined these human public dataset found ITGB1 expression BC patient tumor samples positively correlated grade. Likewise, levels three worse outcomes. This pre-clinical model provides unique opportunity validate recapitulate results discovered studies pursue future mechanistic therapeutic interventions for