Tumour-suppressive microRNA-144-5p directly targets CCNE1 / 2 as potential prognostic markers in bladder cancer
作者： R Matsushita , N Seki , T Chiyomaru , S Inoguchi , T Ishihara
DOI: 10.1038/BJC.2015.195
关键词: Cancer cell 、 Cell 、 Oncology 、 Internal medicine 、 Cancer research 、 Cell cycle 、 Cancer 、 Oncogene 、 microRNA 、 Gene expression 、 Medicine 、 Transfection
摘要: Analysis of a microRNA (miRNA) expression signature bladder cancer (BC) by deep-sequencing revealed that clustered miRNAs (miR)-451a, miR-144-3p, and miR-144-5p were significantly downregulated in BC tissues. We hypothesised these function as tumour suppressors BC. The aim this study was to investigate the functional roles their modulation networks cells. studies cells performed using transfection mature miRNAs. Genome-wide gene analysis, silico dual-luciferase reporter assays applied identify miRNA targets. association between levels target genes determined, overall patient survival estimated Kaplan–Meier method. Gain-of-function showed inhibited cell proliferation Four cycle-related (CCNE1, CCNE2, CDC25A, PKMYT1) identified direct targets miR-144-5p. patients with high CCNE1 or CCNE2 had lower probabilities than those low (P=0.025 P=0.032). functions suppressor directly regulated might be good prognostic markers for patients.
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