Detection and prevention of protein aggregation before, during, and after purification.
作者： Sarah E Bondos , Alicia Bicknell
DOI: 10.1016/S0003-2697(03)00059-9
关键词: Protein aggregation 、 Biochemistry 、 Monomer 、 Protein purification 、 Inclusion bodies 、 Chemistry 、 In vitro 、 FLAG-tag 、 Blot 、 Chromatography 、 Solubility
摘要: … Initial protein concentrations ranged from 3 to 1 mM (see Table 2). The diluted protein was … Aggregated protein retained on the membrane was resuspended in 30 μl of dH 2 O, pipeting …
elsevier.com
sci-hub.se 参考文章(39)
R E Cappel, H F Gilbert, Thiol/disulfide exchange between 3-hydroxy-3-methylglutaryl-CoA reductase and glutathione. A thermodynamically facile dithiol oxidation. Journal of Biological Chemistry. ,vol. 263, pp. 12204- 12212 ,(1988) , 10.1016/S0021-9258(18)37740-8
John E. Coligan, Current Protocols in Protein Science ,(1996)
Serge N. Timasheff, Control of Protein Stability and Reactions by Weakly Interacting Cosolvents: The Simplicity of the Complicated Advances in Protein Chemistry. ,vol. 51, pp. 355- 432 ,(1998) , 10.1016/S0065-3233(08)60656-7
Franz Hofmeister, Zur Lehre von der Wirkung der Salze Naunyn-schmiedebergs Archives of Pharmacology. ,vol. 24, pp. 247- 260 ,(1888) , 10.1007/BF01918191
Kunihiko GEKKO, Tatsuya MORIKAWA, Preferential Hydration of Bovine Serum Albumin in Polyhydric Alcohol-Water Mixtures1 The Journal of Biochemistry. ,vol. 90, pp. 39- 50 ,(1981) , 10.1093/OXFORDJOURNALS.JBCHEM.A133468
Guifu Xie, Serge N. Timasheff, Temperature dependence of the preferential interactions of ribonuclease a in aqueous co‐solvent systems: Thermodynamic analysis Protein Science. ,vol. 6, pp. 222- 232 ,(1997) , 10.1002/PRO.5560060124
Markos I. Moraitis, Han Xu, Kathleen S. Matthews, Ion Concentration and Temperature Dependence of DNA Binding: Comparison of PurR and LacI Repressor Proteins† Biochemistry. ,vol. 40, pp. 8109- 8117 ,(2001) , 10.1021/BI0028643
Jie Chen, Kathleen S. Matthews, Subunit dissociation affects DNA binding in a dimeric lac repressor produced by C-terminal deletion. Biochemistry. ,vol. 33, pp. 8728- 8735 ,(1994) , 10.1021/BI00195A014
Guifu Xie, Serge N. Timasheff, Mechanism of the stabilization of ribonuclease A by sorbitol: preferential hydration is greater for the denatured then for the native protein. Protein Science. ,vol. 6, pp. 211- 221 ,(1997) , 10.1002/PRO.5560060123
M. F. Perutz, B. J. Pope, D. Owen, E. E. Wanker, E. Scherzinger, Aggregation of proteins with expanded glutamine and alanine repeats of the glutamine-rich and asparagine-rich domains of Sup35 and of the amyloid β-peptide of amyloid plaques Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 5596- 5600 ,(2002) , 10.1073/PNAS.042681599