Poly(3-hydroxybutyrate) synthesis in fed-batch culture of Ralstonia eutropha with phosphate limitation under different glucose concentrations.
作者： Longan Shang , Min Jiang , Ho Nam Chang
关键词: Eutropha 、 Biochemistry 、 Poly-3-hydroxybutyrate 、 Phosphate 、 Ralstonia 、 Escherichia coli 、 Economic shortage 、 Fed-batch culture 、 Carbon source 、 Chemistry 、 Food science
摘要: Poly(3-hydroxybutyrate) (PHB) was produced by fed-batch cultures of Ralstonia eutropha with phosphate limitation under different glucose concentrations. When kept at 2.5 g l−1, cell growth and PHB synthesis were limited due to the shortage carbon source but a higher content occurred in cell-growth stage. This shows that low concentration is favorable for accumulation R. eutropha. obtained 9 l−1 1.6 times 40 l−1. range productivity decreased significantly increase concentration. The highest
springer.com
sci-hub.se 参考文章(10)
Genta Kobayashi, Kuniaki Tanaka, Hirokazu Itoh, Takeharu Tsuge, Kenji Sonomoto, Ayaaki Ishizaki, Fermentative production of P(3HB-co-3HV) from propionic acid by Alcaligenes eutrophus in fed-batch culture with pH-stat continuous substrate feeding method Biotechnology Letters. ,vol. 22, pp. 1067- 1069 ,(2000) , 10.1023/A:1005650132371
Sang Yup Lee, Ho Nam Chang, Production of poly(hydroxyalkanoic acid) Advances in Biochemical Engineering \/ Biotechnology. ,vol. 52, pp. 27- 58 ,(1995) , 10.1007/BFB0102315
Jens Nielsen, Gregory N. Stephanopoulos, Aristos A. Aristidou, Metabolic Engineering: Principles and Methodologies ,(1998)
G. Braunegg, B. Sonnleitner, R. M. Lafferty, A rapid gas chromatographic method for the determination of poly- β -hydroxybutyric acid in microbial biomass European Journal of Applied Microbiology and Biotechnology. ,vol. 29, pp. 29- 37 ,(1978) , 10.1007/BF00500854
Alexander Steinbüchel, Bernd Füchtenbusch, Bacterial and other biological systems for polyester production Trends in Biotechnology. ,vol. 16, pp. 419- 427 ,(1998) , 10.1016/S0167-7799(98)01194-9
Beom Soo Kim, Seung Chul Lee, Sang Yup Lee, Ho Nam Chang, Yong Keun Chang, Seong Ihl Woo, Production of poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) by fed-batch culture of Alcaligenes eutrophus with substrate control using on-line glucose analyzer Enzyme and Microbial Technology. ,vol. 16, pp. 556- 561 ,(1994) , 10.1016/0141-0229(94)90118-X
Longan Shang, Min Jiang, Chul Hee Ryu, Ho Nam Chang, Soon Haeng Cho, Jong Won Lee, Inhibitory effect of carbon dioxide on the fed‐batch culture of Ralstonia eutropha: Evaluation by CO2 pulse injection and autogenous CO2 methods Biotechnology and Bioengineering. ,vol. 83, pp. 312- 320 ,(2003) , 10.1002/BIT.10670
Jong-il Choi, Sang Yup Lee, High-Level Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Fed-Batch Culture of Recombinant Escherichia coli Applied and Environmental Microbiology. ,vol. 65, pp. 4363- 4368 ,(1999) , 10.1128/AEM.65.10.4363-4368.1999
B. Xu, M. Jahic, G. Blomsten, S.-O. Enfors, Glucose overflow metabolism and mixed-acid fermentation in aerobic large-scale fed-batch processes with Escherichia coli Applied Microbiology and Biotechnology. ,vol. 51, pp. 564- 571 ,(1999) , 10.1007/S002530051433
Beom-Soo Kim, Kyung-Suk Cho, Hee-Wook Ryu, Hyun-Joo Shim, Ho-Nam Chang, Yong-Keun Chang, Mass production of poly(3-hydroxybutyrate) by fed-batch cultures of Ralstonia eutropha with nitrogen and phosphate limitation Journal of Microbiology and Biotechnology. ,vol. 9, pp. 751- 756 ,(1999)