Poly(3-Hydroxybutyrate) Production in Repeated fed-Batch with Cell Recycle Using a Medium with low Carbon Source Concentration

摘要: Among approaches applied to obtain high productivity and low production costs in bioprocesses are cell density the use of cost substrates. Usually substrates, as waste/agroindustrial residues, have carbon concentration, which leads a difficulty operating bioprocesses. Real time control process for intracellular products is also difficult. The present study proposes strategy repeated fed-batch with recycle attain Cupriavidus necator poly(3-hydroxybutyrate) (P(3HB)) productivity, using substrate source concentration (90 g l(-1)). Also, oxygen uptake rate data was pointed out an on line solution control, once P(3HB) product. results showed that total biomass (X), residual (Xr) values at end culture were 61.6 g l(-1), 19.3 g l(-1) 42.4 g l(-1) respectively, equivalent 68.8 % cells, 1.0 g l(-1) h(-1). Therefore, proposed efficient achieve polymer content from medium concentration.