In vivo thrombolysis with targeted microbubbles loading tissue plasminogen activator in a rabbit femoral artery thrombus model

摘要: The increasingly high incidence of ischemic stroke caused by thrombosis the arterial vessels is one major factors that threaten people's health and lives in world. present treatments for are unsatisfactory yet. We developed microbubbles loading tissue plasminogen activator (tPA) their vitro thrombolysis efficacy under ultrasound exposure has been proved previously. tried to investigate effect vivo this study. Thrombus model was made clamping bilateral femoral arteries 70 40 rabbits. targeted tPA-loaded were lyophilization, taking arginine-glycine-aspartic acid-serine peptide as targeting ligand. Its efficacy, calculated count rate efficiency recanalization, evaluated Pearson's χ(2) One-way ANOVA, respectively. recanalization (70%) similar combination tPA, (80%) (P = 0.61), while its tPA dosage (0.06 mg/kg) much less than latter (0.9 mg/kg). highest among all groups (53.22 ± 40.39%) < 0.01). Ultrasound-induced release a promising thrombolytic method with satisfactory lowered dose potentially decreased hemorrhagic risk.