The Role of Phosphoinositide 3-Kinase C2α in Insulin Signaling

摘要: The members of the class II phosphoinositide 3-kinase (PI3K) family can be activated by several stimuli, indicating that these enzymes regulate many intracellular processes. Nevertheless, to date, there has been no definitive identification their in vivo product, mechanism(s) activation, or precise roles. By metabolic labeling, we here identify phosphatidylinositol 3-phosphate as sole product insulin-dependent activation PI3K-C2, confirming emerging role such a signaling. We demonstrate PI3K-C2 involves its recruitment plasma membrane and is mediated GTPase TC10. This first report showing targeting-mediated mechanism for small GTP-binding protein activate PI3K isoform. also contributes maximal insulin-induced translocation glucose transporter GLUT4 subsequent uptake, definitely assessing this enzyme insulin