Effect of Hyperthermia on CHO DNA Polymerases α and β
作者： Ira J. Spiro , David L. Denman , William C. Dewey
DOI: 10.2307/3575691
关键词: Enzyme 、 Radiochemistry 、 Hyperthermia 、 Alpha (ethology) 、 Cell culture 、 Cell killing 、 Radiosensitivity 、 DNA polymerase 、 Chemistry 、 Incubation 、 Biophysics 、 Radiation 、 Radiology Nuclear Medicine and imaging
摘要: The heat sensitivity of polymerase ..beta.. (pol-..beta..) has been studied in a an attempt to assess the molecular lesions for killing and radiosensitization. Viable cells were heated, then activity was determined crude cell extracts by measuring incorporation (/sup 3/H) TMP into exogenous, activated calf thymus DNA. thermal inactivation pol-..beta.. shown correlate with hyperthermic Pol-..beta.. decreased exponentially when heated at temperatures 43/sup o/C above, 10-min 45.5/sup shock reduced 80%. Also, showed 3-fold greater than ..cap alpha.. (pol-..cap alpha..) 43.5 o/C. When 42.2-42.5/sup o/C, decreased, but as killing, tollerance any further treatment observed after 2 hr heating. Thermal tolerance also decrease rage if given either preconditioning 42.2/sup 6 hr, or 10 min followed 24 37/sup Furthermore, o/Cmore » presence 7 mM procaine-HCl, both loss greatly enhanced. Finally, return correlated radiosensitization evidenced increase survival (10 min, o/C) subsequent X-ray separated increasing amounts time For example, during incubation treatment, logarithm increased over 4-fold, while pol-..cap only 1.4-fold 75% its control activity.« less
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