Dynamics of VEGF matrix-retention in vascular network patterning.

摘要: Vascular endothelial growth factor (VEGF) is a central regulator of blood vessel morphogenesis, although its role in patterning cells into vascular networks not fully understood. It has been suggested that binding soluble VEGF to extracellular matrix components causes spatially restricted cues guide network patterns. Yet, current evidence for such mechanism remains indirect. In this study, we quantitatively analyse the dynamics retention controlled vitro situation human umbilical (HUVECs) Matrigel. We show fluorescent accumulates pericellular areas and colocalizes with molecules. Analysis fluorescence recovery after photobleaching reveals binding/unbinding molecules dominates region. Computational simulations using our experimental measurements kinetic parameters chemotactic signals can lead formation reticular cellular on realistic timescale. Taken together, these results binds proximity HUVECs Matrigel, suggest bound drives patterning.