Near-ultraviolet tyrosyl circular dichroism of pig insulin monomers, dimers, and hexamers. Dipole-dipole coupling calculations in the monopole approximation.

作者: E. Hardin Strickland , Dan Mercola

DOI: 10.1021/BI00662A035

关键词: MonomerCircular dichroismDimerMoleculeDichroismCrystallographyRadiation chemistryIonChemistryRandom hexamer

摘要: The tyrosyl circular dichroism (CD) has been calculated using the conformation of pig insulin observed in rhombohedral crystals containing 2 zinc atoms per hexamer. These calculations predict that CD at 275 nm will be enhanced disproportionally as monomers interact to form dimers and hexamers. This (..delta..epsilon 5800 molecular weight) results from new coupling interactions generated regions contact between dimers. illustrate a large enhancement may accompany aggregation even absence change either monomer. intensities for monomers, dimers, hexamers 2-zinc are compatible with experimentally spectra which about fourfold hexamer compared Zinc ions other metals do not contribute directly but only influence optical properties by promoting hexameric state. relation integrity molecule dimer formation biological activity molecules discussed. largest contributions arise far-ultraviolet transitions neighboring aromatic groups. In hexamer, half themore » intensity is Tyr-A14.« less

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