Proteomic analysis of rat skeletal muscle submitted to one bout of incremental exercise

摘要: Exercise can alter gene transcriptional and protein translational rates leading to changes in abundance toward adaptation exercise. We investigated the alterations skeletal muscle after one bout of an exhaustive exercise through proteomic analysis. Gastrocnemius muscles were sampled from non-exercised control rats exercised on a treadmill with incremental increases speed until exhaustion (approximately 30 min). Rats sacrificed 3 24 h cessation. Two-dimensional gel electrophoresis was performed spots significant alteration relative volume identified by mass spectrometry. Six presented statistically altered abundances The as metabolic related proteins triosephosphate isomerase 1, glyceraldehyde-3-phosphate dehydrogenase, β subunit pyruvate dehydrogenase E(1) carnitine palmitoyltransferase 2 all more abundant One spot heat shock cognate 70 also demonstrated decreased α-actin. These results suggest that single session untrained thin filaments synthesis/degradation rate enhance cytosolic mitochondrial synthesis. may be important general preconditioning for subsequent sessions.