Effects of 1,25(OH)2D3, 25OHD3, and EB1089 on cell growth and Vitamin D receptor mRNA and 1α-hydroxylase mRNA expression in primary cultures of the canine prostate ☆
作者： S. Kunakornsawat , T.J. Rosol , C.C. Capen , J.L. Omdahl , B.E. Leroy
DOI: 10.1016/J.JSBMB.2004.03.009
关键词: Endocrinology 、 Calcifediol 、 Calcitriol receptor 、 Cell growth 、 Calcitriol 、 Gene expression 、 Prostate 、 Internal medicine 、 Vitamin D and neurology 、 Biology 、 Stromal cell
摘要: The aim of this study was to investigate effects 1,25(OH)(2)D(3) (calcitriol), 25OHD(3), and EB1089 on cell growth Vitamin D receptor (VDR) mRNA 1alpha-hydroxylase (1alpha-OHase) expression in normal canine prostatic primary cultures. Canine epithelial cells were isolated, cultured, treated with vehicle (ethanol), calcitriol, at 10(-9) 10(-7)M. VDR present stromal the prostate gland. 1,25(OH)(2)D(3), inhibited 10(-7)M compared vehicle-treated controls [calcitriol (P < 0.01), 25OHD(3) 0.05)]. Epithelial calcitriol had slightly increased (0.2-0.3-fold) 6 12h controls. There no difference 1alpha-OHase these three compounds. its analogs may be effective antiproliferative agents certain types cancer.
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