S-Adenosylmethionine regulates MAT1A and MAT2A gene expression in cultured rat hepatocytes: a new role for S-adenosylmethionine in the maintenance of the differentiated status of the liver

摘要: Methionine metabolism starts with the formation of S-adenosylmethionine (AdoMet), most important biological methyl donor. This reaction is catalyzed by methionine adenosyltransferase (MAT). MAT product two different genes: MAT1A, which expressed only in adult liver, and MAT2A, widely distributed, fetal replaces MAT1A hepatocarcinoma. In preservation high expression low MAT2A critical for maintenance a functional differentiated organ. Here we describe that cultured rat hepatocytes progressively decreased, as described other liver-specific genes, was induced. We find this switch gene prevented adding AdoMet to culture medium. also show decreased addition markedly increased transcription dose-dependent fashion. effect mimicked methionine, blocked 3-deazaadenosine L-ethionine, but not D-ethionine, indicating specific mediated probably methylation reaction. These findings identify key molecule differentially regulates helps maintain status hepatocyte.