Convergence of signaling pathways on the activation of ERK in B cells.

摘要: The B cell receptor (BCR) initiates three major signaling pathways: the Ras pathway, which leads to extracellular signal-regulated kinase (ERK) activation; phospholipase C-gamma causes calcium mobilization; and phosphoinositide 3-kinase (PI 3-kinase) pathway. These combine induce different biological responses depending on context of BCR signal. Both PI pathways are important for development activation. Several model systems show evidence cross-regulation between these pathways. Here we demonstrate through use inhibitors a dominant-negative construct that BCR-induced phosphorylation activation ERK is dependent 3-kinase. feeds into cascade at multiple points, both upstream downstream Ras. We also C-gamma, in keeping with its dependence mobilization. Last, itself completely conclude cascades intimately connected cells signal integration point, since it requires simultaneous input from all