B cell antigen receptor stimulation induces formation of a Shc-Grb2 complex containing multiple tyrosine-phosphorylated proteins.
作者： J. Borst , L. Smit , A. M. M. De Vries-Smits , J. L. Bos
DOI: 10.1016/S0021-9258(17)31975-0
关键词: SH2 domain 、 Receptor tyrosine kinase 、 Tyrosine phosphorylation 、 Cell surface receptor 、 Molecular biology 、 ROR1 、 Platelet-derived growth factor receptor 、 Protein tyrosine phosphatase 、 Biology 、 Tyrosine kinase
摘要: Activation of growth factor receptor tyrosine kinases, such as the epidermal and insulin receptors, induces phosphorylation Shc proteins their association with SH2 domain-containing adaptor protein Grb2. The Shc-Grb2 complex has been implicated in coupling these receptors to p21ras. B cell antigen plays a key role directing proliferation differentiation. Although lacks intrinsic kinase activity, its mode action parallels that kinases many aspects. stimulation activates src-related syk, which leads various cytoplasmic initiates multiple signaling events, including p21ras activation. Therefore, we have investigated whether are targets for activated receptor. It was found 52- 46-kDa forms expressed mature human cells become rapidly phosphorylated on upon stimulation. Also, is induced associate Grb2 molecule an undefined 130-kDa protein. In specific response activation, associates several tyrosine-phosphorylated proteins, two prominent phosphoproteins molecular masses 130 110 kDa. These observations strongly suggest involved one or signal transduction pathways.
sci-hub.st 参考文章(30)
J C Cambier, A E Harwood, B cell antigen receptor cross-linking triggers rapid protein kinase C independent activation of p21ras1. Journal of Immunology. ,vol. 151, pp. 4513- 4522 ,(1993)
B. M. Burgering, R. H. Medema, J. A. Maassen, M. L. van de Wetering, A. J. van der Eb, F. McCormick, J. L. Bos, Insulin stimulation of gene expression mediated by p21ras activation. The EMBO Journal. ,vol. 10, pp. 1103- 1109 ,(1991) , 10.1002/J.1460-2075.1991.TB08050.X
C. W. Turck, A. L. Defranco, M. R. Gold, V. W.-F. Chan, Membrane Ig cross-linking regulates phosphatidylinositol 3-kinase in B lymphocytes. Journal of Immunology. ,vol. 148, pp. 2012- 2022 ,(1992)
J.E. Hutchcroft, M.L. Harrison, R.L. Geahlen, B lymphocyte activation is accompanied by phosphorylation of a 72-kDa protein-tyrosine kinase. Journal of Biological Chemistry. ,vol. 266, pp. 14846- 14849 ,(1991) , 10.1016/S0021-9258(18)98549-2
G. M. W. Van Schijndel, J. Borst, R. A. W. Van Lier, J. L. Cordell, A. G. D. Tse, C. J. M. Vna Noesel, D. Y. Mason, E. F. R. De Vries, The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. Journal of Immunology. ,vol. 146, pp. 3881- 3888 ,(1991)
A L DeFranco, M R Gold, M T Crowley, G A Martin, F McCormick, Targets of B lymphocyte antigen receptor signal transduction include the p21ras GTPase-activating protein (GAP) and two GAP-associated proteins. Journal of Immunology. ,vol. 150, pp. 377- 386 ,(1993)
Maria Rozakis-Adcock, Ross Fernley, John Wade, Tony Pawson, David Bowtell, The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1 Nature. ,vol. 363, pp. 83- 85 ,(1993) , 10.1038/363083A0
Sean E. Egan, Barton W. Giddings, Mary W. Brooks, László Buday, Andrew M. Sizeland, Robert A. Weinberg, Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation Nature. ,vol. 363, pp. 45- 51 ,(1993) , 10.1038/363045A0
Giuliana Pelicci, Luisa Lanfrancone, Francesco Grignani, Jane McGlade, Federica Cavallo, Guido Forni, Ildo Nicoletti, Fausto Grignani, Tony Pawson, Pier Giuseppe Pelicci, A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction. Cell. ,vol. 70, pp. 93- 104 ,(1992) , 10.1016/0092-8674(92)90536-L
X. R. Bustelo, M. Barbacid, Tyrosine Phosphorylation of the vav Proto-Oncogene Product in Activated B Cells Science. ,vol. 256, pp. 1196- 1199 ,(1992) , 10.1126/SCIENCE.256.5060.1196