A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction.

摘要: A new SH2-containing sequence, SHC, was isolated by screening cDNA libraries with SH2 representative DNA probes. The SHC is predicted to encode overlapping proteins of 46.8 and 51.7 kd that contain a single C-terminal domain, an adjacent glycine/proline-rich motif regions homology the alpha 1 chain collagen, but no identifiable catalytic domain. Anti-SHC antibodies recognized three 46, 52, 66 in wide range mammalian cell lines. These complexed were phosphorylated activated epidermal growth factor receptor. physical association receptors recreated vitro using bacterially expressed NIH 3T3 mouse fibroblasts constitutively overexpressed acquired transformed phenotype culture formed tumors nude mice. results suggest gene products couple signaling pathway regulates proliferation cells.