In-vitro morphogenesis of corn (Zea mays L.) : I. Differentiation of multiple shoot clumps and somatic embryos from shoot tips.

摘要: In-vitro methods have been developed to regenerate clumps of multiple shoots and somatic embryos at high frequency from shoot tips aseptically-grown seedlings as well apices precociously-germinated immature zygotic corn (Zea mays L.). About 500 were produced a tip after eight weeks culture (primary one subculture four weeks) in darkness on Murashige Skoog basal medium (MS) supplemented with mg/L casein hydrolysate (CH) 9 μM N6-benzyladenine (BA). In this medium, formed tightly packed “multiple clumps” (MSC), which composed some axillary many adventitious shoots. When the cultured MS containing CH, BA 2.25 2,4-dichlorophenoxyacetic acid (2,4-D), most origin. Similar 4.5 2,4-D regenerated within culture. Somatic either directly apical meristems or calli derived apices. Both MSC normal 1.8 indole-3-butyric (IBA). These rooted 3.6 IBA, fertile plants grown greenhouse. The sweet-corn genotype, Honey N Pearl, was used for experiments described above, but shoot-tip cultures all 19 other genotypes tested also CH BA.