Analysis of microbial community in Japanese vineyard soils by culture-independent molecular approach

摘要: Soil microbes play an important role in the management of soil environment. They form various microbial communities response to environmental factors, including texture and chemical components. Because this, it is difficult determine community structure soil. In vineyard soils, overall has yet be unraveled. To understand soil, we surveyed comprehensively Japanese soils by using a culture-independent molecular approach. We identified 681 fungal clone sequences 1076 bacterial samples collected from nine independent vineyards, results suggested that Ascomycota dominant group community, whereas Proteobacterium Acidobacterium are groups community. DNA was directly extracted samples, internal transcribed spacer 1 (ITS 1) region or 16S ribosomal (rDNA) amplified polymerase chain reaction. The recovered clones were sorted into 225 operational taxonomic units majority assigned Ascomycota. Meanwhile, 17 phyla, abundant phyla Acidobacterium. These differed reported structures forest agricultural soils. Moreover, preliminarily generated catalog extremely complex, suggesting each individual characteristics. Our study showed for first time most likely provide clue nature