V‐REVCOMP: automated high‐throughput detection of reverse complementary 16S rRNA gene sequences in large environmental and taxonomic datasets

摘要: Reverse complementary DNA sequences - that are inadvertently given backwards with all purines and pyrimidines transposed can affect sequence analysis detrimentally unless taken into account. We present an open-source, high-throughput software tool -v-revcomp (http://www.cmde.science.ubc.ca/mohn/software.html) to detect reorient reverse entries of the small-subunit rRNA (16S) gene from sequencing datasets, particularly environmental sources. The supports lengths ranging full length down short reads characteristic next-generation technologies. evaluated reliability v-revcomp by screening 406 781 16S deposited in release 102 curated SILVA database demonstrated has a detection accuracy virtually 100%. subsequently used analyse 1 171 646 International Nucleotide Sequence Databases found about 1% these user-submitted were complementary. In addition, nontrivial proportion otherwise anomalous, including chimeras, associated wrong taxa, nonribosomal genes, poor quality or erroneous without reasonable match any other entry database. Thus, is highly efficient detecting reorienting almost be various anomalies.