Hepatitis delta virus genome replication: a polyadenylated mRNA for delta antigen.
作者： S Y Hsieh , M Chao , L Coates , J Taylor
DOI: 10.1128/JVI.64.7.3192-3198.1990
关键词: Biology 、 Molecular biology 、 Protein biosynthesis 、 Hepatitis delta Antigens 、 Viral replication 、 RNA polymerase II 、 Virus 、 Messenger RNA 、 Virology 、 Polyadenylation 、 RNA
摘要: Hepatitis delta virus (HDV) replicates its genome in the nucleus of an infected cell. However, unsolved problem has been identification cytoplasm a putative mRNA for synthesis only virus-coded protein, antigen. We now report characterization 800-base RNA that is cytoplasmic, polyadenylated, and antigenomic should direct translation This was about 500 times less abundant than full-length genomic RNA. mapped predominant 5' terminus also 3' site at which poly(A) added. At point 15 to 20 bases upstream addition sequence AAUAAA, could have used as signal polyadenylation. When infectious cDNA clone whole HDV changed this UUUAAA, no longer it unable These findings provided additional evidence polyadenylated least method expression Apparently processed if were host polymerase II transcript, although did not necessarily indicate transcribed with enzyme.
sci-hub.st 参考文章(34)
Kang-Sheng Wang, Qui-Lim Choo, Amy J Weiner, Jing-Hsiung Ou, Richard C Najarian, Richard M Thayer, Guy T Mullenbach, Katherine J Denniston, John L Gerin, Michael Houghton, None, Structure, sequence and expression of the hepatitis delta ( δ ) viral genome Nature. ,vol. 323, pp. 508- 514 ,(1986) , 10.1038/323508A0
Philip Bernstein, Jeffrey Ross, Poly(A), poly(A) binding protein and the regulation of mRNA stability Trends in Biochemical Sciences. ,vol. 14, pp. 373- 377 ,(1989) , 10.1016/0968-0004(89)90011-X
Brenda L. Bass, Harold Weintraub, Roberto Cattaneo, Martin A. Billeter, Biased hypermutation of viral RNA genomes could be due to unwinding/modification of double-stranded RNA Cell. ,vol. 56, pp. 331- ,(1989) , 10.1016/0092-8674(89)90234-1
John M Chirgwin, Alan E Przybyla, Raymond J MacDonald, William J Rutter, None, Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. ,vol. 18, pp. 5294- 5299 ,(1979) , 10.1021/BI00591A005
A. Kos, R. Dijkema, A. C. Arnberg, P. H. van der Meide, H. Schellekens, THE HEPATITIS DELTA (DELTA) VIRUS POSSESSES A CIRCULAR RNA Nature. ,vol. 323, pp. 558- 560 ,(1986) , 10.1038/323558A0
M.R. Rebagliati, D.A. Melton, Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity Cell. ,vol. 48, pp. 599- 605 ,(1987) , 10.1016/0092-8674(87)90238-8
B BASS, A developmentally regulated activity that unwinds RNA duplexes. Cell. ,vol. 48, pp. 607- 613 ,(1987) , 10.1016/0092-8674(87)90239-X
Patrick J. O'Hara, Stuart T. Nichol, Frank M. Horodyski, John J. Holland, Vesicular stomatitis virus defective interfering particles can contain extensive genomic sequence rearrangements and base substitutions Cell. ,vol. 36, pp. 915- 924 ,(1984) , 10.1016/0092-8674(84)90041-2
A K Banerjee, Transcription and replication of rhabdoviruses. Microbiological Research. ,vol. 51, pp. 66- 87 ,(1987) , 10.1128/MR.51.1.66-87.1987
M. A. Frohman, M. K. Dush, G. R. Martin, Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 8998- 9002 ,(1988) , 10.1073/PNAS.85.23.8998