Surface plasma modification and tropoelastin coating of a polyurethane co-polymer for enhanced cell attachment and reduced thrombogenicity
作者： Daniel V Bax , Alexey Kondyurin , Anna Waterhouse , David R McKenzie , Anthony S Weiss
DOI: 10.1016/J.BIOMATERIALS.2014.04.082
关键词: Polymer 、 Biocompatibility 、 Tropoelastin 、 Human umbilical vein endothelial cell 、 Dermal fibroblast 、 Biophysics 、 Materials science 、 Extracellular matrix 、 Coating 、 Thrombogenicity 、 Composite material
摘要: Polymers currently utilized for dermal and vascular applications possess sub-optimal biocompatibility which reduces their efficacy. Improving the cell-binding blood-contacting properties of these polymers would substantially improve clinical utility. Tropoelastin is a highly extensible extracellular matrix protein with beneficial cell interactive low thrombogenic properties. We transferred benefits to polyurethane block copolymer Elast-Eon E2A through specific combination surface plasma modifications coating human tropoelastin. The activity bound tropoelastin was modulated by ion implantation underlying polymer, correlated hydrophobicity, carbon oxygen content. This combined treatment enhanced fibroblast (HDF) umbilical vein endothelial (HUVEC) attachment, cytoskeletal assembly viability, elevated PECAM-1 staining HUVEC junctions. thrombogenicity polymer ameliorated coating. propose that metered can serve biological performance implantable devices such as conduits.
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