Free radical functionalization of surfaces to prevent adverse responses to biomedical devices

作者: M. M. M. Bilek , D. V. Bax , A. Kondyurin , Y. Yin , N. J. Nosworthy

DOI: 10.1073/PNAS.1103277108

关键词:

摘要: Immobilizing a protein, that is fully compatible with the patient, on surface of biomedical device should make it possible to avoid adverse responses such as inflammation, rejection, or excessive fibrosis. A strongly binds and does not denature protein required. Hydrophilic surfaces do induce denaturation immobilized but exhibit low binding affinity for protein. Here, we describe an energetic ion-assisted plasma process can any hydrophilic at same time enable covalently immobilize functional biological molecules. We show modification creates free radicals migrate from reservoir beneath. When they reach surface, form covalent bonds biomolecules. The kinetics number densities molecules in solution control required full monolayer bound. shelf life capability governed by initial density depth reservoir. high reactivity renders universal across all macromolecules. Because radical be created solid material, this approach used medical applications ranging cardiovascular stents heart-lung machines.

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