The linker-free covalent attachment of collagen to plasma immersion ion implantation treated polytetrafluoroethylene and subsequent cell-binding activity.

摘要: It is desirable that polymers used for the fabrication of prosthetic implants promote biological functions such as cellular adhesion, differentiation and viability. In this study, we have plasma immersion ion implantation (PIII) to modify surface polytetrafluoroethylene (PTFE), thereby modulating binding mechanism collagen. The amount collagen bound polymer following PIII-treatment was similar by non-covalent physisorption. a manner consistent with previous enzyme tropoelastin data, PIII-treated PTFE resistant sodium dodecyl sulfate (SDS) elution whilst untreated fully removed. This demonstrates capability surfaces covalently attach without employing chemical linking molecules. Only supported human dermal fibroblast attachment spreading. indicates on possesses increased adhesive activity compared surface. Cell adhesion inhibited EDTA when PTFE, expected integrin involvement. Additionally sensitive conformation Increased actin cytoskeletal assembly observed cells spreading onto collagen-coated PTFE. These data demonstrate retention collagen's properties its suggesting advantages tissue engineering design.