Plasma‐Treated Polyethylene Surfaces for Improved Binding of Active Protein
作者： Joan P. Y. Ho , Neil J. Nosworthy , Marcela M. M. Bilek , Bee K. Gan , David R. McKenzie
关键词: Polyethylene 、 Enzyme 、 Enzyme assay 、 Biochemistry 、 Nuclear chemistry 、 Plasma-immersion ion implantation 、 Wetting 、 Horseradish peroxidase 、 Chemistry 、 Plasma protein binding 、 Immobilized enzyme
摘要: The use of plasma immersion ion implantation (PIII) to prepare polyethylene surfaces for binding active proteins is investigated. PIII surface treatment significantly improves the density HRP bound after incubation in buffer containing protein. retention enzyme activity on PIII-treated greatly improved as compared both untreated controls and exposed without treatment. A nitrogen produced with best protein activity. Oxidation ion-damaged formation a carbonized subsurface are believed be responsible observed properties.
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