In vitro expression of N-acetyl aspartate by oligodendrocytes: implications for proton magnetic resonance spectroscopy signal in vivo.

摘要: Abstract: Magnetic resonance spectroscopy (MRS) provides a noninvasive means of assessing in vivo tissue biochemistry. N-Acetyl aspartate (NAA) is major brain metabolite, and its presence used increasingly clinical experimental MRS studies as putative neuronal marker. A reduction NAA levels assessed by 1H has been suggested to be indicative viability. However, temporal observations pathologies such multiple sclerosis, mitochondrial encephalopathy with lactic acidosis stroke-like episodes (MELAS), hypothyroidism have shown reversibility levels, possibly reflecting recovery function. knowledge the cellular localisation critical interpreting these findings. The assumption that specific neurones based on previous immunohistochemical whole using NAA-specific antibodies. was further substantiated cell culture experiments which oligodendrocyte-type 2 astrocyte progenitors immature oligodendrocytes, but not mature observed. More recently, oligodendrocyte biology revealed requirement for trophic factors promote generation, maturation, survival oligodendrocytes vitro. Here, we this new information implement more pertinent cultivation procedure demonstrate can express This observation brings into question whether changes observed reflect function alone. data presented here support hypothesis may contribute signal MRS.