A method to measure the radio and chemosensitivity of human spheroids.

摘要: A method based on the spontanous outgrowth of cells from spheroids was tested. Different patterns were seen depending types and radiation or drug doses. The allowed dose-effect relations to be determined. Spheroid survival defined as when outgrowing monolayers contained at least thousand within five weeks. used an alternative cloning isolated single cells. gliana osteosarcoma could not disintegrated cell suspensions since they resisted enzymatic mechanical treatments for separation. Detection differences in radio chemosensitivity between different human origin might valuable understanding large variations therapeutical response often tumors.