Simple in vitro migration assay for neural crest cells and the opposite effects of all-trans-retinoic acid on cephalic- and trunk-derived cells.
作者： Makoto Usami , Katsuyoshi Mitsunaga , Tomohiko Irie , Atsuko Miyajima , Osamu Doi
DOI: 10.1111/CGA.12059
关键词: Cell biology 、 Internal medicine 、 Embryo culture 、 In vitro 、 Embryo 、 Biology 、 Migration Assay 、 Tretinoin 、 Trunk 、 Retinoic acid 、 Endocrinology 、 Neural crest
摘要: Here, we describe a simple in vitro neural crest cell (NCC) migration assay and the effects of all-trans-retinoic acid (RA) on NCCs. Neural tubes excised from rhombencephalic or trunk region day 10.5 rat embryos were cultured for 48 h to allow emigration Migration NCCs was measured as change radius (radius ratio) calculated circular spread between 24 culture. RA added culture medium after 24 h at embryotoxic concentrations determined by whole embryo (10 μM) reduced cephalic NCCs, whereas it enhanced indicating that has opposite these two types
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