Proteomic analysis of selenium embryotoxicity in cultured postimplantation rat embryos.
作者： Makoto Usami , Katsuyoshi Mitsunaga , Ken Nakazawa , Osamu Doi
DOI: 10.1002/BDRB.20145
关键词:
摘要: BACKGROUND: Developmental toxicity of selenium (Se) is a nutritional, environmental and medicinal concern. Here, we investigated Se embryotoxicity by proteomic analysis cultured rat embryos. METHODS: Rat embryos at day 9.5 or 10.5 gestation were for 48 24 h, respectively, in the presence sodium selenate (100 150 µM) selenite (20 30 µM). Proteins from embryo proper yolk sac membrane analyzed two-dimensional electrophoresis quantitative changes those control with identified mass spectrometric analysis. RESULTS: Growth inhibition morphological abnormalities observed all treatment groups. By proper, actin-binding proteins as selenate: increased phosphorylated-cofilin 1, phosphorylated-destrin, decreased drebrin E, myosin light polypeptide 3. Many showed similar between selenite, including ATP-synthase, acidic ribosomal phosphoprotein P0, pyrroline-5-carboxylate reductase-like. In membrane, antioxidant protein spots selenite: peroxiredoxin 1 glutathione S-transferase. CONCLUSION: The considered to be candidate involved embryotoxicity: embryotoxicity, common modification redox-related treatments. These may also used biomarkers developmental studies. Birth Defects Res (Part B), 2008. © 2008 Wiley-Liss, Inc.
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