Molecular typing and antifungal susceptibility of Trichophyton rubrum isolates from patients with onychomycosis pre- and post-treatment

摘要: Forty sequential isolates of Trichophyton rubrum were obtained from patients suffering onychomycosis at two time points, before and after antifungal oral therapy. Strain differentiation by specific amplification the tandemly repeated elements (TRS-1 TRS-2) ribosomal DNA T. was performed. In addition, susceptibility tests executed microdilution method with nine drugs: ketoconazole, itraconazole, fluconazole, miconazole, clotrimazole, isoconazole, griseofulvin, cyclopiroxolamine terbinafine. The combination TRS-1 TRS-2 PCR patterns configured 11 genotypes three most prevalent (genotypes 1-I, 5-I 2-I) accounted for 67.5% isolates. Seven (35%) therapy exhibited genotype 1-I compared to (55%) treatment. Twelve different strains With respect in vitro testing, terbinafine potent agent, followed cyclopiroxolamine, griseofulvin fluconazole. Furthermore, an increase minimum inhibitory concentrations (MIC) observed azole agents when testing post-treatment four patients. This MIC occurred concomitantly major occurrence These data attempt consider relevance vivo drug resistance caused rubrum.