Variation in restriction fragment length polymorphisms among serial isolates from patients with Trichophyton rubrum infection.

摘要: Molecular genotyping of strains Trichophyton rubrum and T. mentagrophytes from patients with onychomycosis the toes was performed to ascertain whether fungal genotype changes over course time as sequential samples were obtained receiving antifungal therapy during follow-up. Sixty-six serial 11 20 (16 rubrum, 4 mentagrophytes) who treated oral observed periods up 36 months. These screened for genetic variation by hybridization EcoRI-digested genomic DNAs a probe amplified small-subunit (18S) ribosomal DNA adjacent internal transcribed spacer regions. A total five restriction fragment length polymorphism (RFLP) types among 66 rubrum. Two major RFLP types, differentiated one band shift, represented 68% samples. None had unique genotype. More than type often single patient (same nail) period 1, 2, or 3 years, even in cases that did not appear cured at any time. Samples taken different nails same either The genotypic correspond detectable phenotypic variation. Furthermore, no correlation between efficacy treatment administered observed. While region studied distinguished mentagrophytes, tonsurans, intraspecific strains. independent multiple infection coinhabitation may explain presence genotypes nail, microevolutionary events such rapid substrain shuffling, seen studies repetitive regions Candida species, also produce result. recovery sampling uncured further suggests typing system is able distinguish relapse reinfection, ongoing infection, de novo infection.