Osteopontin Promotes Invasion, Migration and Epithelial-Mesenchymal Transition of Human Endometrial Carcinoma Cell HEC-1A Through AKT and ERK1/2 Signaling
作者： Yinghua Li , Yunpeng Xie , Dan Cui , Yanni Ma , Linlin Sui
DOI: 10.1159/000438518
关键词: Cancer 、 Cell biology 、 Cancer cell 、 Angiogenesis 、 Extracellular matrix 、 Osteopontin 、 Metastasis 、 Biology 、 Protein kinase B 、 Epithelial–mesenchymal transition
摘要: Background/Aims: Osteopontin (OPN) is an Extracellular Matrix (ECM) molecule and involved in many physiologic pathologic processes, including cell adhesion, angiogenesis tumor metastasis. OPN a well-known multifunctional factor various aspects of cancer progression, endometrial cancer. In this study, we examined the significance Methods: The proliferation, migration invasion ability HEC-1A cells were detected by Cell Counting Kit-8 (CCK-8), Wound scratch assay transwell. Western blots employed to detect expression metalloproteinase-2 (MMP-2) epithelial-mesenchymal transition (EMT)-related factors treated with rhOPN. Results: rhOPN promotes cells. influenced EMT-related MMP-2 promoted migration, EMT through protein kinase B (PKB/AKT) regulated kinases (ERK1/2) signaling pathway. Conclusions: These results may open up novel therapeutic strategy for cancer: namely, have important roles controlling growth suggest target pathway treatment
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