Regulation of the pehA gene encoding the major polygalacturonase of Xanthomonas campestris by Clp and RpfF
作者： Yi-Min Hsiao , Mei-Huei Zheng , Rouh-Mei Hu , Tsuey-Ching Yang , Yi-Hsiung Tseng
DOI: 10.1099/MIC.0.2007/012930-0
关键词: Microbiology 、 Gene 、 Catabolite repression 、 Transcription (biology) 、 Biochemistry 、 Virulence 、 Endopeptidase Clp 、 Xanthomonas campestris 、 Biology 、 Enoyl-CoA hydratase 、 Rapid amplification of cDNA ends
摘要: Exopolysaccharide and several extracellular enzymes of Xanthomonas campestris pv. (Xcc), the causative agent black rot in crucifers, are virulence factors. In this study, sequence mutational analysis has demonstrated that Xcc pehA encodes major polygalacturonase, a member family 28 glycosyl hydrolases. Using 5' RACE (rapid amplification cDNA ends) method, transcription initiation site was mapped at 102 nt downstream Clp (cAMP receptor protein-like protein)-binding site. Transcriptional fusion assays showed is greatly induced by polygalacturonic acid, positively regulated RpfF (an enoyl-CoA hydratase homologue which required for synthesis cis-11-methyl-2-dodecenoic low-molecular-mass diffusible signal factor), subjected to catabolite repression, independent or RpfF, repressed under conditions oxygen limitation nitrogen starvation. Our findings extend previous work on regulation show they both influence expression Xcc.
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