First molecular phylogeny of Agrilus (Coleoptera: Buprestidae), the largest genus on Earth, with DNA barcode database for forestry pest diagnostics.

摘要: All more than 3000 species of Agrilus beetles are phytophagous and some cause economically significant damage to trees shrubs. Facilitated by international trade, regularly invade new countries continents. This necessitates a rapid identification species, as the first step for subsequent protective measures. study provides DNA reference library ~100 from Northern Hemisphere based on three mitochondrial markers: cox1–5′ (DNA barcode fragment), cox1–3′ , rrnL . 329 records available in Barcode Life Database format, including specimen images geo data, released through public dataset ‘Agrilus1 329’ at: dx.doi.org/10.5883/DS-AGRILUS1. were identified using adult morphology molecular phylogenetic trees, well distance- tree-based algorithms. Most DNA-based limits agree with morphology-based identification. Our results include cases high intraspecific variability multiple para- polyphyly. barcoding is powerful tool although it frequently fails recover morphologically-delimited species-group. Even though current three-gene database covers only ~3% known diversity, contains representatives all principal lineages represents most extensive built DNA-delimited within this genus so far. Molecular data analyses can rapidly cost-effectively identify an unknown sample, immature stages and/or non-native taxa, or not yet formally named.