Isolation of the C-type lectin like-domain cDNAs from the mud crab Scylla paramamosain Estampador, 1949, and its expression profiles in various tissues, during larval development, and under Vibrio challenge
作者： K. Jiang , D. Zhang , M. Sun , L. Ma , L. Qi
关键词: Vibrio parahaemolyticus 、 Hepatopancreas 、 Complementary DNA 、 C-type lectin 、 Biochemistry 、 Biology 、 Scylla paramamosain 、 Vibrio 、 cDNA library 、 Anatomy 、 Lectin
摘要: Scylla paramamosain Estampador, 1949 is one of the most precious marine crabs farmed in China. The crab prone to infection by microbes at various stages during its growth, leading high mortality. C-type lectin has a characteristic carbohydrate recognition domain and plays an important role immunity system. A cDNA library from mud S. was constructed using SMART technique. Two complete sequences showing identities with gene were isolated library. two full-length cDNAs (SpLec1 SpLec2) consist 746 834 bp, respectively. Quantitative realtime PCR analyses revealed that lectins expressed mainly haemocytes, muscle hepatopancreas, highest expression level found hepatopancreas. mRNA continuous developmental zoeal phases also examined quantitative real-time PCR. After Vibrio parahaemolyticus (Sakazaki, 1963) concentration 2.3 × 10 6 cfu/ml, temporal SpLec1 SpLec2 megalopa stage first increased, reached maximum, then dropped original again. research could shed new light on studies moulting crab.
brill.com
brillonline.com参考文章(29)
Xian-Wei Wang, Xiao-Wen Zhang, Wen-Teng Xu, Xiao-Fan Zhao, Jin-Xing Wang, A novel C-type lectin (FcLec4) facilitates the clearance of Vibrio anguillarum in vivo in Chinese white shrimp Developmental & Comparative Immunology. ,vol. 33, pp. 1039- 1047 ,(2009) , 10.1016/J.DCI.2009.05.004
Kenneth Söderhäll, Lage Cerenius, Role of the prophenoloxidase-activating system in invertebrate immunity Current Opinion in Immunology. ,vol. 10, pp. 23- 28 ,(1998) , 10.1016/S0952-7915(98)80026-5
H KONG, E PARK, B NAM, Y KIM, W KIM, H PARK, C LEE, S LEE, A C-type lectin like-domain (CTLD)-containing protein (PtLP) from the swimming crab Portunus trituberculatus. Fish & Shellfish Immunology. ,vol. 25, pp. 311- 314 ,(2008) , 10.1016/J.FSI.2008.05.003
Alex N Zelensky, Jill E Gready, The C-type lectin-like domain superfamily FEBS Journal. ,vol. 272, pp. 6179- 6217 ,(2005) , 10.1111/J.1742-4658.2005.05031.X
Frida Soria, Claudia Sierra, Stephane Bouquelet, Colette Brassart, Concepción Agundis, Edgar Zenteno, Lorena Vázquez, The effect of sugars and free amino acids from the freshwater prawn Macrobrachium rosenbergii hemolymph on lectin activity and on oxidative burst Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. ,vol. 142, pp. 212- 219 ,(2006) , 10.1016/J.CBPC.2005.10.003
Tracy Hoi-Tung Ma, John A.H. Benzie, Jian-Guo He, Siu-Ming Chan, PmLT, a C-type lectin specific to hepatopancreas is involved in the innate defense of the shrimp Penaeus monodon. Journal of Invertebrate Pathology. ,vol. 99, pp. 332- 341 ,(2008) , 10.1016/J.JIP.2008.08.003
Masahiro Hosono, Shigeki Sugawara, Yukiko Ogawa, Takayuki Kohno, Motoaki Takayanagi, Kazuo Nitta, Purification, characterization, cDNA cloning, and expression of asialofetuin-binding C-type lectin from eggs of shishamo smelt (Osmerus [Spirinchus] lanceolatus) Biochimica et Biophysica Acta. ,vol. 1725, pp. 160- 173 ,(2005) , 10.1016/J.BBAGEN.2005.07.009
Tracy Hoi Tung Ma, Shirley Hiu Kwan Tiu, Jian-Guo He, Siu-Ming Chan, Molecular cloning of a C-type lectin (LvLT) from the shrimp Litopenaeus vannamei: Early gene down-regulation after WSSV infection Fish & Shellfish Immunology. ,vol. 23, pp. 430- 437 ,(2007) , 10.1016/J.FSI.2006.12.005
P.S. Gross, T.C. Bartlett, C.L. Browdy, R.W. Chapman, G.W. Warr, Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific White Shrimp, Litopenaeus vannamei , and the Atlantic White Shrimp, L. setiferus Developmental & Comparative Immunology. ,vol. 25, pp. 565- 577 ,(2001) , 10.1016/S0145-305X(01)00018-0
Kunihiro Yamaura, Keisuke G. Takahashi, Tohru Suzuki, Identification and tissue expression analysis of C-type lectin and galectin in the Pacific oyster, Crassostrea gigas. Comparative Biochemistry and Physiology B. ,vol. 149, pp. 168- 175 ,(2008) , 10.1016/J.CBPB.2007.09.004