Effect of shear stress in the flow through the sampling needle on concentration of nanovesicles isolated from blood

摘要: Abstract During harvesting of nanovesicles (NVs) from blood, blood cells and other particles in are exposed to mechanical forces which may cause activation platelets, changes membrane properties, cell deformation shedding fragments. We report on the effect shear imposed upon samples during process, concentration isolates blood. Mathematical models flow through needle sampling with vacuumtubes free were constructed, starting Navier–Stokes formalism. Blood was modeled as a Newtonian fluid. Work stress calculated. In experiments, isolated by repeated centrifugation (up 17,570 × g) washing, counted cytometry. It found that positively corresponded work sample needle. have enhanced shaking prior isolation glass beads. Imaging scanning electron microscopy revealed closed globular structures similar size shape those obtained unshaken plasma repetitive washing. Furthermore, sizes shapes NVs erythrocytes shaken platelet-rich platelet rich plasma, not induced exogenously added amphiphiles. These results favor hypothesis significant pool is created their harvesting. The identity, shape, composition strongly depend technology