Isoelectric focusing and crossed immunoelectrofocusing of CSF immunoglobulins in MS
作者： �. Sid�n
DOI: 10.1007/BF00313168
关键词: Immunoglobulin G 、 Immunoglobulin light chain 、 Molecular biology 、 Immunology 、 Antibody 、 Isoelectric focusing 、 Gamma globulin 、 Immunoglobulin A 、 Chemistry 、 Multiple sclerosis 、 Immunoglobulin M
摘要: The 3 main Ig classes and the presence of free light chains were studied by isoelectric focusing crossed immunoelectrofocusing in 100 CSF samples from patients with clinically definitie or probable MS. Minute quantities IgM light-chain (mostly lambda) components found 2 out 11 5 14 respectively. IgG IgA detected all examined for these proteins pI-ranges 5.3-9.8 4.6-6.4 pH-units gammaglobulin abnormalities on identified as microheterogeneous, oligoclonal predominantly kappa determinants. immunoprecipitates differed those normal subjects major abnormal most frequently exhibited pI-values greater than 8 pH-units. had 2-4 some tendency to discontinuous subfractionation. This class, however, did not exhibit marked distribution IgG.
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