Study of biofilm formation by total-reflection X-ray fluorescence spectrometry

作者: K. Kröpfl , Gy. Záray , P. Vladár , M. Mages , É. Ács

DOI: 10.1016/S0026-265X(03)00088-2

关键词: Surface waterChemical decompositionX-ray fluorescenceElemental analysisChemistryQuantitative analysis (chemistry)Environmental chemistryBiomonitoringSample preparationBiofilm

摘要: Abstract A total reflection X-ray fluorescence spectrometric method was developed for elemental analysis of natural biofilms grown on polycarbonate substrates in the Lake Velence. For duration 9 weeks long growing period, two were removed weekly from lake and investigated by analytical algological methods. The biomass production achieved its highest value after 7 weeks. Ca, Sr Ti, as well Fe, Mn, K Zn showed their maximum concentrations 5 6–7 weeks, respectively. enrichment factors 6 old biofilm detected seven elements amounted to 10 3 –10 4 . recommended colonization time biomonitoring Velence is applying substrates.

参考文章(12)
F. G. Ferris, S. Schultze, T. C. Witten, W. S. Fyfe, T. J. Beveridge, Metal Interactions with Microbial Biofilms in Acidic and Neutral pH Environments. Applied and Environmental Microbiology. ,vol. 55, pp. 1249- 1257 ,(1989) , 10.1128/AEM.55.5.1249-1257.1989
W Admiraal, H Blanck, M Buckert-de Jong, H Guasch, N Ivorra, V Lehmann, B.A.H Nyström, M Paulsson, S Sabater, Short-term toxicity of zinc to microbenthic algae and bacteria in a metal polluted stream Water Research. ,vol. 33, pp. 1989- 1996 ,(1999) , 10.1016/S0043-1354(98)00426-6
Kyle D. Hoagland, James R. Rosowski, Michael R. Gretz, Stephen C. Roemer, DIATOM EXTRACELLULAR POLYMERIC SUBSTANCES: FUNCTION, FINE STRUCTURE, CHEMISTRY, AND PHYSIOLOGY Journal of Phycology. ,vol. 29, pp. 537- 566 ,(1993) , 10.1111/J.0022-3646.1993.00537.X
Michael C. Newman, Alan W. McIntosh, Appropriateness of Aufwuchs as a monitor of bioaccumulation. Environmental Pollution. ,vol. 60, pp. 83- 100 ,(1989) , 10.1016/0269-7491(89)90222-4
Zs. Czégény, B. Berente, M. Óvári, M.Garcı́a Tapia, Gy. Záray, Microwave-Assisted Vapor-Phase Acid Digestion of Cellulose Nitrate Filters for Elemental Analysis of Airborne Dust Samples Microchemical Journal. ,vol. 59, pp. 100- 106 ,(1998) , 10.1006/MCHJ.1998.1568
K Friese, M Mages, K Wendt-Potthoff, T.R Neu, Determination of heavy metals in biofilms from the River Elbe by total-reflection X-ray fluorescence spectrometry Spectrochimica Acta Part B: Atomic Spectroscopy. ,vol. 52, pp. 1019- 1025 ,(1997) , 10.1016/S0584-8547(96)01633-3
J. W. G. Lund, C. Kipling, E. D. Le Cren, The inverted microscope method of estimating algal numbers and the statistical basis of estimations by counting Hydrobiologia. ,vol. 11, pp. 143- 170 ,(1958) , 10.1007/BF00007865
Ian W. Sutherland, Biofilm exopolysaccharides: a strong and sticky framework. Microbiology. ,vol. 147, pp. 3- 9 ,(2001) , 10.1099/00221287-147-1-3